![]() ![]() ‘With the Gel Doc EZ imaging system, we’ve made imaging easy without sacrificing quality or accuracy,’ said Ryan Short, marketing manager for Imaging Systems at Bio-Rad Laboratories. This eliminates the need for researchers to manipulate manually filters, lenses, or lighting, thereby minimising the potential for human error, the company says. The Gel Doc EZ imager requires no training for first-time users and provides automated push-button functionality. The system is for researchers who perform DNA, RNA, and protein electrophoresis as well as western blotting. ![]() The Gel Doc EZ imager from life sciences supplier Bio-Rad Laboratories delivers publication-quality images and gel analysis results in seconds - with just the push of a button. Furthermore, stain-free technology enables the user to validate western blotting data by total protein normalization rather than the use of housekeeping genes or stripping and reprobing, yielding quantitative results rather than relative normalization.Easy imaging without sacrificing quality or accuracy The Bio-Rad ChemiDoc and Gel Doc Gel Documentation Systems are stain-free enabled and can be used to rapidly check the quality of gel electrophoresis and transfer efficiency. Stain-free gels display Laemmli-like separation characteristics and are compatible with standard sample and running buffers. Stain-free gels can be visualized in less than 5 minutes after electrophoresis and again after blotting. The use of a gel documentation system to rapidly evaluate gels after electrophoresis, and membranes after transfer, saves reagents and time. Gel Documentation Systems Enabled for Stain-Free Gels ![]() In addition to increased accuracy, multifluorescent detection saves time, and fewer reagents are used. A gel imaging system that is capable of multifluorescent detection increases accuracy because there is no error due to inaccuracy in replicate loading or the loss of protein that occurs during stripping a blot for reprobing. This permits the detection of multiple proteins in a single sample, without requiring loading the same sample in multiple wells or stripping and reprobing the blot. Multifluorescent western blot detection allows the simultaneous detection of more than one protein on a blot. SAM provides fast, optimal results for ECL and other chemiluminescence-based protocols. In gel imaging systems with SAM capability, the system determines the best exposure time and captured image. SAM enables the capture and analysis of a time course of the development of chemiluminescent signals, unlike film-based systems, where an image is captured at a single arbitrary time point. Signal accumulation mode (SAM) is the collection of chemiluminescence data over time, allowing the measurement of chemiluminescence as it develops. Specific software modules are also offered to satisfy 21 CFR Part 11 regulatory requirements. For analyses that are often repeated, automation of the specific data acquisition, analysis, and output modes can save time and reduce errors. Multiple modes of data analysis and output provide ease of data evaluation, customized reports, sharing of data, different types of output, and generation of publication-quality images. Additionally, as your needs change and expand, our gel documentation systems have the flexibility to accommodate different sample types and detection modes. Modular design and flexible options allow you to customize a system for your specific requirements. What features might you need in a gel documentation system? Bio-Rad's gel documentation systems accommodate a wide range of samples and support different modes of detection. The choice of a gel documentation system depends on both your current and anticipated future needs. Most Bio-Rad gel documentation systems also feature our unique stain-free imaging technology, allowing rapid visualization of proteins on gels and blots without staining and destaining (Bio-Rad's TGX Stain-Free or Criterion Stain Free Precast Gels, or TGX Stain-Free FastCast Acrylamide Solutions, are also required). Our gel imaging systems can be used for detection and automated data analysis with all common modes of protein and nucleic acid staining and labeling: colorimetric, fluorescent, chemiluminescent, and radioisotopic. These gel documentation systems have advanced software, providing rapid automated data acquisition, analysis, and validation. Bio-Rad offers a comprehensive range of imaging systems for the detection, quantitation, and analysis of proteins and nucleic acids in gels and on membranes. ![]()
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